Hello! I am
Florian Schüder
About Me
I am a Physicist venturing into Biology.
My scientific passion is the understanding of interactions between biomolecules at all levels of life.
I studied Physics at the Ludwig-Maximilians University of Munich and the Wyss Institute of Harvard University from 2009 – 2015. Afterward, I pursued a Ph.D. in Biophysics at the Max Planck Institute of Biochemistry in Martinsried from 2015-2020. Since 2021 I am a Postdoctoral Fellow in the group of Joerg Bewersdorf and Jorge E. Galán at Yale University.
Ph.D. Projects
Advancing and applying Next-Generation DNA-based super-resolution microscopy.
Universal multiplexing by DNA Exchange
Unlimited multiplexing for dye based fluorescence microscopy.
Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT
Whole cell imaging with DNA-PAINT.
Quantifying absolute addressability in DNA origami with molecular resolution
Staple incorporation into DNA origami on a single-molecule level.
Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically‐Encoded Probes for DNA‐PAINT
SNAP-Tag, Halo-Tag and Nanobodies as labeling probes for DNA-PAINT.
An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions
10 x Faster DNA-PAINT.
Postdoc Projects
Super‐resolution spatial proximity detection with proximity‐PAINT
Super-resolved proximity detection with a detection range of 0 nm to 20 nm.
Nanobodies combined with DNA-PAINT super-resolution reveal a staggered titin nanoarchitecture in flight muscles
Investigation of the spatial organization of the two Titin homologs in Drosophila flight muscle.
Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging
Fast, efficient, gentle, and unlimited multiplexed super-resolution imaging with FLASH-PAINT.
Smart probe microscopy fueled by the molecular programmability of DNA.
Imaging Pearls
Three phase separated compartments of the nucleolus. Granular component (here NPM1) in green, dense fibrillar component (here FBL) in red and fibrillar core (here RPA40) in blue.
Salmonella (red) uses its flagellum to attack a dividing (blue = DNA) eukaryotic cell. The eukaryotic cell starts ruffling (green = actin) upon the attack.
Two color DNA-PAINT super-resolution image. Microtubules are presented in yellow and the mitochondria network is displayed in magenta.
The mitochondria network of a COS-7 cell. The color represents the axial position.
Actin cytoskeleton of a COS-7 cell. Spatial resolution ~10 nm.
The ER network of a U-2 OS cell.